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The peroxisome proliferator-activated receptor r(PPARr), a member of the steroid/thyroid nuclear hormone receptor suferfamily of ligand-activated transcription factor, is an important regulator of adipocyte gene expression and differentiation. In this studies, we report the identification, characterization, and expression of a Hanwoo PPARr gene. The PPARr cDNA sequence of the Hanwoo show strong conservation with the corresponding sequences reported in other species except of three amino acid sequences. The distribution of PPARr mRNA in various tissues of Korean cattle aged 12 months were investigated using Northern Blot analysis. The higher expression was detected in adipose tissue, more lower expression was detected in colon, small intestine, kidney, lung, while expression was not detected in brain, heart. PPAR? expression was higher in adipose tissue of Korean cattle when aged 30 months than aged 12 months. These results indicated PPARr, regulator adipocyte gene expression and differentiation, related on adipose differentiation in Korean native cattle(HANWOO).
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